D-Lactate Assay Kit (EFDLC-100)

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-Lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems' EnzyFluoTM lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at ex/em = 530/585 nm, is proportional to the lactate concentration in the sample. This product is for Research Use only and is not intended for diagnostic purposes.

• For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects.
• Method: Fluorometric assay (530/585 nm). A spectrophotometer or plate reader is required, but not provided.
• Samples: Serum, plasma, cell culture media etc. Species: All. Detects 1 uM
• Procedure: 60 min. Size: 100 tests per kit